PromoCell's 3D Tumorsphere Medium XF

The next generation 3D Tumorsphere Medium XF from PromoCell has been designed to meet your requirements for the extended serial 3D tumorsphere culture. The 3D Tumorsphere Medium supports the tumorsphere/mammosphere culture of the most commonly used cancer cell lines.

Setting a New Standard with Next Generation Tumorsphere Medium

Key Advantages:

  • Easy 3D tumorsphere culture system
  • Directly switch from 2D to 3D culture
  • Supports most commonly used cancer cell lines
  • Allows extended serial tumorsphere passages (>10)
  • High tumorsphere formation efficiency
  • Xeno-free and serum-free defined formulation
  • Ready to use

The PromoCell 3D Tumorsphere Medium XF

The PromoCell 3D Tumorsphere Medium XF has been designed to meet your requirements for the extended serial 3D tumorsphere culture. This 3D Tumorsphere Medium XF supports the most commonly used cancer stem cell lines in tumorspheres/mammospheres culture. In contrast to the current adherent 2D culture of cancer cells, this type of 3D culture selectively exploits inherent biologic features of CSC, such as anoikis resistance and self-renewal. Continuous proliferation is also supported during serial passage of 3D tumorsphere cultures (see Fig. below). Thus, this culture system is also applicable for in vitro models of metastasis. PromoCell’s 3D Tumorsphere Medium XF is ready-to-use and xeno-free, providing a standardized culture devoid of stimuli of uncharacterized origin. This is a significant benefit in terms of CSC which are a population of highly responsive stem cells requiring reliable and reproducible control of the selfrenewal/differentiation axis. The xeno-free PromoCell 3D Tumorsphere Medium XF is suitable for the cost-efficient and standardized routine culture of tumorspheres/mammospheres for a wide range of cell lines.


Proliferation and tumorsphere formation efficiency (TFE) of MCF-7 cells during serial passage in 3D tumorsphere culture using the PromoCell 3D Tumorsphere Medium XF. Robust proliferation was maintained during the culture, which was discontinued after passage 10 with no sign of growth rate inhibition. Serial passage in the 3D Tumorsphere Medium XF resulted in significant increase of TFE from 2% in P1 to 14% in P4, and 28% in P9, respectively.


 
Passage 10 serial tumosphere culture of HT1080 fibrosarcoma cells using the 3D Tumorsphere Medium XF.
 Passage 10 serial tumosphere culture of MCF-7 mammary carcinoma cells using the 3D Tumorsphere Medium XF.

List of cell types tested for serial passage with the PromoCell 3D Tumorsphere Medium XF

Tissue
Tested Cell Lines
Cell Lines Origin
Brain
U-87 MG
grade IV glioblastoma / astrocytoma of the human brain
Breast
MCF-7
pleural effusion of metastatic human breast adenocarcinoma
Colon
HT-29
human colon adenocarcinoma
Connective tissue
HT1080
human fibrosarcoma
Liver
HepG2
hepatocellular carcinoma of the human liver
Lung
A-549
human lung carcinoma
Pancreas
Panc-1
epithelioid carcinoma of the human pancreatic duct
Prostate
LNCaP
lymph node metastasis of human prostate adenocarcinoma
Skin
A-431
epidermoid carcinoma of the human skin

Products

Cat. No.Description
AmountBrand
C-28070
3D Tumorsphere Medium XF
250 ml
PromoCell
C-28075
3D Tumorsphere Medium XF, phenol red-free
250 ml
PromoCell
Related products
C-40232
Dulbecco’s PBS, w/o Ca++/Mg++
500 ml
PromoCell
C-41210
DetachKit-125
3 x 125 ml
PromoCell



Source:
Website: PromoCell 3D Tumorsphere Medium XF
Application Note: Tumorsphere Culture of Cancer Stem Cells (CSC) with the PromoCell 3D Tumorsphere Medium XF, Determination of the Tumorsphere Formation Efficiency (TFE) with the PromoCell 3D Tumorsphere Medium XF

Related to:
Brands: PromoCell
Product groups: (Cell) Culture
​News: Primary Cancer Culture System