PD-1 Pathway

PD-1, also known as PDCD1 and CD279, is an immunomodulatory receptor present on the surface of activated T cells and other immune cells. PD-1 has two ligands, PD-L1 (B7-H1, CD274) and PD-L2 (B7-DC, CD273), which are upregulated in most human cancers. Binding of P D-L1 to PD-1 negatively regulates T cell signaling and inhibits cytotoxic T cell activity, allowing the cancer cells to escape immune detection. The PD-1/PD-L pathway is also a therapeutic target for chronic viral-infections including HIV and HCV. PD-1 expression is upregulated on virus-specific T cells, and blockade of this system is hoped to decrease viral load by increasing T cell function. Additionally, the PD-1/PD-L interaction regulates self-tolerance and immunologic homeostasis, making these proteins promising drug targets for many autoimmune disorders including multiple sclerosis, arthritis, lupus, and type I diabetes.

PD1 pathway proteins

BPS offers high purity recombinant extracellular domains of PD-1, PD-L1, and PD-L2 proteins. These proteins are also available biotinylated for detection assays.

Purity and gel image for PD-1 (BPS #71106) and biotinylated PD-L1 (#71105).

Cat. No.DescriptionAmount
71106PD-1 (PDCD1), Fc Fusion
100 ug
71107PD-L2 (CD273), Fc Fusion100 ug
71108PD-L2 (CD273), Biotin-Labeled
50 ug

Assay Kits

These four convenient assay kits are designed to screen monoclonal antibody or small molecule antagonists of PD-1 binding to either of the PD-L ligands. The kits are optimized for high-throughput screening applications using chemiluminescence detection.

(Left) PD-1:PD-L1 binding activity, measured with the PD-1:PD-L1[Biotinylated] Inhibitor Screening Assay Kit, Catalog #72003 and (Right) Inhibition of PD-1:PD-L1 binding by PD-L2, measured using the same kit. 

Cat. No.DescriptionAmount
72003PD-1:PD-L1[Biotinylated] Inhibitor Screening Assay Kit96 rxns
72004PD-1:PD-L2[Biotinylated] Inhibitor Screening Assay Kit96 rxns
72005PD-1[Biotinylated]:PD-L1 Inhibitor Screening Assay Kit96 rxns
72006PD-1[Biotinylated]:PD-L2 Inhibitor Screening Assay Kit96 rxns

Related to:
Brands: BPS Bioscience
Product groups: AssaysProteins / Signaling Molecules