LentiBrite RFP-LC3 Lentiviral Biosensor

Catalog number: 17-10143
Brand: Merck
Packing: 1 Vials
Price: € 560.00
Expected delivery time: 7 days
Quantity:

Product specifications for - LentiBrite RFP-LC3 Lentiviral Biosensor

Overview: 
Product group: DNA / RNA / Vectors
Category: Plasmid / vector
Properties: 
  Research Use Only
UNSPSC: 41106600
Storage instructions: Storage and HandlingLentivirus is stable for at least 4 months from date of receipt when stored at -80-¦C. After first thaw, place immediately on ice and freeze in working aliquots at -80-¦C. Frozen aliquots may be stored for at least 2 months. Further freeze/thaws may result in decreased virus titer and transduction efficiency. IMPORTANT SAFETY NOTEReplication-defective lentiviral vectors, such as the 3rd Generation vector provided in this product, are not known to cause any diseas
Scientific information: 
Scientific info: Autophagy, a degradative pathway that provides recycled nutrients to cells under stress, plays both protective and deleterious roles in many diseases, including cancer, neurodegeneration, and infections. Members of the LC3 family play a key role in the maturation of the autophagosome, the central organelle of autophagy. LC3 precursors, diffusely distributed in the cytosol, are proteolytically processed to form LC3-I. Upon initiation of autophagy, the C-terminal glycine is converted to LC3-II by addition of a phosphatidylethanolamine by Atg5-Atg12. LC3-II translocates rapidly to nascent autophagosomes in a punctate distribution. DNA constructs encoding fluorescent proteins fused to LC3 are widely employed for introduction into cells for monitoring autophagosome formation by fluorescence microscopy. EMD Millipore’s LentiBrite RFP-LC3 lentiviral particles provide bright fluorescence and precise localization to enable live cell analysis of autophagy in difficult-to-transfect cell types.
Additional information: 
Synonyms: 17-10143; Merck (Millipore)