Reagents for Animal Feed Analysis

Megazyme has recognised the need for simple and specific enzyme assay kits and reagents for the measurement of enzymes, carbohydrates and anti-nutritional components in animal feeds.

Nutritional Qualities of Feed

Grains and legume seeds are major components of feed formulations and contain a range of anti-nutrients. Examples include phytic acid and dietary fiber components such as b-glucan, arabinoxylan, cellulose, resistant starch and raffinose-oligosaccharides (ROS). These anti-nutrients are detrimental to the optimal digestibility of feed by animals and need to be measured accurately. The addition of appropriate enzymes to feed degrades these anti-nutrients, thereby improving the digestibility of the feed and increase its nutritional value.

Megazyme has developed accurate and specific methods and substrates for the measurement of the nutrients, anti-nutrients and key enzymes in feed.

Total starchK-TSTA assay kit
Frutose / Glucose K-FRUGL assay kit

beta-GlucanK-BGLU assay kit
ArabinoxylanK-XYLOSE assay kit
Resistant StarchK-RSTAR assay kit
PhytateK-PHYT assay kit

Xylanase Xylazyme & Xylazyme AX tabletsK-XylX6 assay kit
beta-Glucanasebeta-glucazyme tabletsK-MBG4 assay kit
alpha-AmylaseAmylazyme tabletsCeralpha assay kit
CellulaseCellazyme tabletsK-CellG5 assay kit

Megazyme is revolutionizing how enzymes are measured with the launch of its new range of colourimetric oligosaccharide substrates

In the past, enzyme measurement was performed using reducing sugar enzyme assays (eg. DNSA) employing polysaccharides as substrates. In the 1980s, reducing sugar assays in the feed industry were replaced with procedures involving Megazyme’s dyed polysaccharide substrates (Xylazyme, b-Glucazyme, Amylazyme etc). Tablet tests are extremely useful for the rapid, convenient and accurate assays of important enzymes such as xylanase, b-glucanase, a-amylase etc.

Despite the huge advantages of tablet tests, enzyme measurements obtained with them must always be related back to the benchmark ‘reference method’ which is based on reducing sugar assays.

Megazyme’s new XylX6 assay for the measurement of xylanase will surely become the new industrial ‘reference method’ given its many advantages over the traditional reducing sugar method.

Better still!
Megazyme have developed similar assay kits for all of the important polysaccharide degrading enzymes found in animal feed.

alpha-Amylase (K-CERA, K-AMYLSD)endo-Cellulase (K-CellG5)
beta-Glucanase / Lichenase (K-MBG4)endo-Xylanase (K-XyLX6

AnalyteCat. No.Analyte SignificanceAdvantages Of Megazyme Test Kits

Important Feed Analytes

Acetic AcidK-ACETRMCommonly found in feed or fermented feedK-ACETRM is a rapid, manual assay kit employing AK and phosphotransacetylase. Stable reagents
AmmoniaK-AMIARCommonly found in feed or fermented feedRapid reaction rate (~ 3 min at room temperature). Ideal for manual and auto-analyser applications. Stable reagents
L-Lactic AcidK-LATECommonly found in fermented feedRapid reaction, stable reagents. Ideal for manual and autoanalyser applications
Phytic AcidK-PHYTFound in most plant materials. Major form of bound phosphate in plant materialsNovel procedure. Rapid reaction, stable reagents


α-AmylaseK-CERAA key enzyme in most feeds and plant productsNovel assay employing a defined oligosaccharide substrate. High sensitivity and specificity. AOAC Method 2002.01; AACC Method 22-02.01; ICC Standard No. 303; RACI Standard Method; CCFRA Flour Testing Working Group Method 0018
K-MBG4β-Glucanase in feedNovel assay employing a defined oligosaccharide substrate. High sensitivity, specificity and stability. Rapid reaction, ideal for manual and auto-analyser applications
K-MBGLCellulase and β-glucanase levels in feedsOnly kit available. Stable reagents. RACI Standard Method
T-BGZβ-Glucanase in feedRapid reaction. Sensitive. Stable reagent
K-XylX6β-Xylanase in feedRapid, colourimetric, assay with excellent sensitivity and reproducibility. Suitable for use with automated analysis systems.
T-XAXβ-Xylanase in feedSensitive. Easy to use. Stable reagent
ProteaseS-AZCASendo-Protease added to feedEasy to use. Stable reagent

Non-Digestible Carbohydrates

FructanK-FRUCFructo-oligosaccharides in grasses and grainsOnly kit available. Stable reagents; AOAC Method 999.03; AACC Method 32-32.01
GalactomannanK-GALMReserve carbohydrate in many legume seedsOnly kit available, stable reagents
β-Glucan (Barley and oats)K-BGLUMajor cell-wall polysaccharide of barley and oatsRapid reaction, stable reagents, only enzymatic kit available. AOAC Method 995.16; AACC Method 32-23.01; EBC Methods 3.10.1, 4.16.1 and 8.13.1; ICC Standard No.166; RACI Standard Method
Raffinose / D-GalactoseK-RAFGAFound in high levels in legume seeds. Causes discomfort and flatulence in pigsRapid reaction, stable reagents
Resistant StarchK-RSTARStarch that is not digested in the small intestine of monogastric animalsOnly kit available. Stable reagents; AOAC Method 2002.02; AACC Method 32-40.01
Total Dietary Fiber
K-TDFRCarbohydrate not digested in small intestine
1. K-TDFR: AOAC Methods 985.29, 991.42, 991.43 & 993.19; AACC Methods 32-05.01, 32-06.01, 32-07.01 & 32-21.01.
K-INTDF2. K-INTDF is consistent with the CODEX Alimentarius definition of dietary fiber. AOAC Methods 2009.01 & 2011.25; AAAC Methods 32-45.01 & 32-50.11

Digestible Carbohydrates

Available Carbohydrates / Dietary FiberK-ACHDFRapidly and slowly available sugars for digestion or fermentationNovel procedure, stable reagents
D-Fructose / D-Glucose
K-FRUGLMajor digestible carbohydrates in feeds
Rapid reaction times, choice of simple formats available, ideal for manual and auto-analyser applications, stable reagents
Total Starch
K-TSTAStarch content of grain and feed
Rapid assay formats with options of measuring D-glucose with GOPOD reagent or with hexokinase / G-6-PDH. Stable reagents. AOAC Method 996.11; AACC Method 76-13.01; ICC Standard No. 168; RACI Standard Method

Related to:
Brands: Megazyme
Product groups: Assays