Viral antigen bank

Sino Biological launches the world largest recombinant viral antigen collection, ProVir™, which includes over 800 products from 45 different types, 350 strains of viruses. The product line features a wide range of high quality recombinant proteins expressed in insect and mammalian cells. These products are rigorously tested for their purity and bioactivity.

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Highly pure neuron cultures

We welcome BrainXell in our portfolio. They provide high quality neurons from human induced pluripotent stem cells for CNS research and drug discovery.

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New methods for host cell protein analysis

The journal BioPharm International recently spoke with Ken Hoffman, president of Cygnus Technologies, about best practices and new technologies for host cell protein detection and identification as well as the value of outsourcing process-related impurity analytics.

Read the BioPharm Q&A (PDF file) »»

Unique first aid kit for corona prevention

Now that the immediate threat of extreme infection by the coronavirus has passed in our region, most people are picking up their normal lives as much as possible. Meanwhile, we continue to update our COVID-19 pages to contribute to research on treatments and vaccines. In addition, we launch a unique First Aid Kit for Corona Prevention. This kit can come in handy when adjusting your workplace to prevent catching the virus.

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Discover 15 things about cell culture

As a researcher working with cell culture, you surely have a deep understanding of your special area of expertise. However, this field of research is enormous and there is a long cell culture history. PromoCell - Human Centered Science presents some little-known facts that can add to your knowledge.

Read the blog »»

Recombinant Antibodies for SARS-CoV-2 research

The four S1 clones (SARS-CoV-2 spike recombinant rabbit monoclonal antibodies) are specific to SARS-CoV-2 and show no cross-reactivity to SARS-CoV and MERS-CoV spike proteins. Clones [HL1] and [HL6] exhibit high affinity for mammalian-expressed SARS-CoV-2 spike protein. Additionally, they provide SARS-CoV-2 nucleocapsid recombinant rabbit monoclonal antibodies. Although there is high homology among coronavirus nucleocapsid proteins, the clone [HL344] is specific for SARS-CoV-2 with no cross-reactivity to MERS-CoV or SARS-CoV nucleocapsid.

ELISA pairs for Spike protein: many customers are asking for suggested ELISA pairs for Spike protein. GeneTex provides the best combinations as ELISA pairs. The clones in the pairs are also available separately.

Watch the recap of the SARS-CoV-2 and COVID-19 webinar "A world problem needing a world response" from GeneTex’s speaker and senior scientist Alexander Ball, M.D.

Read more on the GeneTex website.

Unused equipment for charity

In this difficult time of corona, we were approached by We-Collect for donating any unused ICT equipment. They collect this to make it possible to set up more home workplaces. In this way the chance of the disease spreading is reduced. Fortunately, we were able to meet their demand. We are very pleased that we, together with We-Collect, have been able to contribute to this charity.

Viral innate sensor

Recently, the lab of Prof. Siddharth Balachandran shows in CELL that the protein ZBP1 detects a certain form of viral RNA (Z-form) in the nucleus triggering the kinase MLKL1, consequently leading to nuclear enveloppe disruption, DNA leaking and necroptosis.
They used anti-ZBP1, mAb (Zippy-1) (Prod. No. AG-20B-0010) from AdipoGen Life Sciences in their studies, confirming the specificity of Zippy-1 in mouse (ZBP1 KO mice) and human cells. Reference.

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Webinar on COVID-19 associated assays and reagents

BioVendor will share information about their assays and reagents and explain what is their utility in the context of COVID-19 outbreak. From diagnosis through research to diagnostic device development. Key points covered in this webinar:

• COVID-19 diagnosis, prognosis and therapy monitoring / disease progression
• Research and assay development reagents

When and Where: April 21st - 09:30 am CET

Join the webinar

Is exosome purity important to you?

Exo-spin mini-HD is the ideal solution for purifying exosomes from intermediate volumes. Moreover, the fractionation profile allows for the selection of individual elution fractions to better control of purity and yield.

Exosomes have specific biophysical properties that allow for a multitude of isolation methods. Size Exclusion Chromatography is ideal to achieve highly purified samples in which exosomes are separated from other non-EV components.

Watch the video on how to use Cell Guidance Systems' latest exosome isolation column Exo-spin mini-HD.

Reliable solution for SARS-CoV-2 detection

Diagnostic providers need access to quality 1-step RT-qPCR reagents to enable an efficient response to the COVID-19 outbreak. The Solis BioDyne production team is managing the surge in demand for SOLIScript 1-step Probe Kit and SOLIScript 1-step Multiplex Probe Kit. These are great products to detect RNA viruses like SARS-CoV-2.

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SARS-CoV-2 cell entry blocked by a clinically proven protease inhibitor

As described in their recent Cell study, Hoffmann et al. identified the serine protease TMPRSS2 as a critical factor in the priming of the SARS-CoV-2 spike protein with its receptor ACE2. They demonstrated that a clinically proven protease inhibitor, camostat mesylate, is able to interfere with SARS-CoV-2 infection of lung cells.

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Coronavirus 2019-nCoV antigen reagents

The ongoing pneumonia outbreak in the city of Wuhan is caused by a newly identified coronavirus 2019-nCoV, named by the World Health Organization on January 12th, 2020. Sino Biological successfully expressed 2019-nCoV spike proteins to develop therapies or further research the virus. This new virus belongs to the Betacoronavirus Genus, which also includes SARS CoV (2003) and MERS CoV (2012).

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ABclonal new in our portfolio

We now offer all products from ABclonal, a company aiming to improve the precision of life science research with their high-quality biology reagents and services. ABclonal provides antibodies, proteins, ELISA kits, NGS library preparation kits and more.

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Mitochondria antibodies

Mitochondrial dysfunction has been linked to natural processes such as aging in addition to being a key factor in a plethora of neurodegenerative, cardiovascular, metabolic, autoimmune, and neoplastic pathologies that plague humans. GeneTex offers a selection of high quality antibodies against mitochondrial markers for research in this area.

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PromoCell's laboratory training courses for 2020

The experienced lecturers at the PromoCell Academy are there to provide you with state-of-the-art insight and up to date trends in the Life Sciences. The seminar center in Heidelberg has extensive training areas and S1 laboratories suited for intensive theoretical and practical work.

» See all English courses for 2020

Three case studies: antibodies as valuable tools in clinical research

The essential role of antibodies in advancing scientific knowledge is supported by thousands of publications. Atlas Antibodies presents three interesting examples that show how antibodies could impact patient survival and wellbeing specifically.

» Discover how antibodies help progressing science [PDF file]

15 year promotion!

To celebrate our 15th anniversary, we are giving away an iPad mini 5 to one of our subscribers. It arrived at our office and is waiting to be unboxed!

The winner will be announced in week 51 on our LinkedIn page. Stay tuned.

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Antibodies to advance veterinary science research

VetSignal™ antibodies will greatly facilitate studies involving animal health, food security, veterinary disease model and diagnostics development, animal welfare and reproduction, etc.

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New: Isolation of exosomes from intermediate volumes

The Exo-spin™ mini-HD column from Cell Guidance Systems is the ideal choice for purifying high-quality exosomes from intermediate volumes.

• High-resolution protocol to optimize yield and purity
• Robust, simple, quick, and economical
• Size Exclusion Chromatography (SEC) method for excellent exosome recovery

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FastGro™: Defined FBS replacement

MP Bio has launched FastGro™, a fully chemically defined FBS replacement for cell culture. It ensures:

• No lot-to-lot variations
• No animal or human derived materials or compounds
• No interference with hormones or growth factors
• Elimination of the risk of contaminants – viruses, mycoplasma, prions, etc.
• Wide range of cell culture practices
• Storage in the refrigerator – no need for thawing

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Spin-columns for protein extraction and cell fractionation

We have added a new supplier to our portfolio, Invent Biotechnologies. They are the inventor of spin column-based disruptive technologies for protein research. Invent offers a smart and simple approach to sample preparation. See how easy protein extraction can be in their video.

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New Product: anti-mouse IL-27 p28

The MM27.7B1 monoclonal antibody reacts with the p28 subunit of mouse IL-27, a member of the IL-6/IL-12 cytokine family. IL-27 is a heterodimeric cytokine composed the EBI3 (Epstein-Barr virus induced gene 3) protein and the p28 subunit, also known as IL-30. Myeloid cells including macrophages, inflammatory monocytes, microglia, and dendritic cells are the dominant cellular sources of IL-27. These cells secrete IL-27 in response to TLR ligands and inflammatory cytokines. IL-27 p28 signals through IL-27 receptors (IL-27R) which are highly expressed by NK cells and activated T cells and to a lesser extent on B cells, naïve T cells, and others. IL-27 signaling has been shown to have both pro-inflammatory and anti-inflammatory effects. Signaling through IL-27R promotes Th1 polarization and IFNγ production but also suppresses the differentiation and proliferation of Th2 and Th17 cells and induces the expression of IL-10. The MM27.7B1 clone has been shown to neutralize the bioactivity of mouse IL-27 in vivo and in vitro and reported to also bind and inhibit human IL-27.

This antibody is useful for:
• in vivo IL-27 p28 neutralization
• in vitro IL-27 p28 neutralization
• Flow Cytometry

Remember to also order the recommended isotype control and dilution buffer.
Click here to request a quote in case of interest.

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Meet a new DNA transfection reagent: Helix-IN™

OZ Biosciences revolutionizes polymer-based transfection with the design of a novel patented Cationic Hydroxylated Amphiphilic Multi-block Polymer (CHAMP™ Technology).
Helix-IN has been designed for DNA delivery to a broad-spectrum of cell lines, preferentially adherent - such as HEK-293, NIH-3T3, CHO, COS, HeLa...

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White Paper: Antibody Affinity Extraction™ (AAE™) Empowers HCP Identification by Mass Spectrometry

Monitoring Host Cell Proteins (HCPs) that co-purify with biological drug substances (DS) is generally done with ELISA, though this method does not provide much insight in the types of HCPs in the sample. Mass Spectometry (MS) allows to identify and quantify HCPs and is used as a complementary method to ELISA. However, the HCPs are often masked by DS. This study describes the use of Antibody Affinity Extraction (AAE) before MS to enrich HCPs, resulting in identification of unknown HCPs and strong general enrichment of the proteins.

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Bullet Blender Tissue Homogenizer

The Bullet Blender® from Next Advance enables you to homogenize, disrupt or lyse up to 24 samples at a time. Load the samples into standard polypropylene tubes, then place them in the Bullet Blender. The "bullets" in the instrument vigorously strike the entire sample and homogenize your sample in minutes. The Bullet Blender homogenizer Gold models keep samples at 4°C!

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Join us at the Foodvalley Summit (17 October)

Meet us at the 3rd international edition of the Foodvalley Summit: The Protein Plan(et) in Wageningen. We supply products for food analysis, GMO and safety tests useful in any laboratory. Other focus area’s include metabolism, obesity and diabetes research. Food Valley Society members benefit from special discounts.

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Bioworld Technology

We now offer all products from Bioworld Technology. They manufature highly purified monoclonal and polyclonal antibodies, peptides and proteins that can be used in all biological fields.

Bioworld Technology is one of the leading phospho-antibody manufacturers. They have produced over 500 phospho-antibodies for AKT, AMPK, GSK, STAT pathways.

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Webinar: Neuron differentiation and transplantation

Join the live webinar with dr. Petr Baranov organized by Cell Guidance Systems about “Improving neuron differentiation and transplantation outcome with slow-release growth factors” on October 10th.

Register here.

Also check out this new publication about PODS, the crystal shell for sustained protein release.

Cancer Cell Line Medium XF

The PromoCell Cancer Cell Line Medium XF is a cell culture medium developed for the standardized in vitro cultivation of established human cancer cell lines. Its serum-free and xeno-free formulation provides a culture environment devoid of all stimuli originating from non-defined materials.

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MedChemExpress "Master of small molecules"

MedChemExpress (MCE) has a comprehensive portfolio of high quality research chemicals and biochemicals including novel life-science reagents, reference compounds, APIs and natural compounds for laboratory and scientific use. They can provide most in-stock products as free samples (0.5-1 mg each). Simply submit the application for the product you need.

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Biochemicals by AdipoGen Life Sciences

AdipoGen Life Sciences offers Swiss quality biochemicals. Their chemical portfolio can be used in the areas of immunology, cancer, obesity and diabetes, inflammation and stem cell research. All products are available from stock and are always highly pure. Many compounds in the portfolio are unique or difficult to extract from natural sources, such as rare antibiotics and biologically active small molecules.

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FUJIFILM Wako Chemicals and Shibayagi ELISA kits 

FUJIFILM Wako Chemicals offers a wide range of world-renowned products in their three business divisions: Laboratory chemicals including analytical reagents and fine chemicals. Specialty chemicals such as azo initiators for radical polymerization, inhibitors for the prevention of polymerization, initiators as well as surfactants and cleaning solutions. Diagnostic reagents and tests are the result of more than 90 years of experience and dedication to the field of in vitro diagnostics. You can purchase these products as ready-to-use kits and/or as bulk.

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BioVision products supplied by Bio-Connect

BioVision offers innovative tools for accelerating drug discovery and biological research. They develop assay kits, antibodies, recombinant proteins and enzymes that can be used for a broad range of studies, such as apoptosis, food science, obesity and metabolic syndrome, stem cell biology, etc. Check out their top products here.

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Need for FBS or animal component free replacement?

Of all the different types of animal serum available, Foetal Bovine Serum (FBS) is the most widely used basal media supplement for in vitro cell culture. Bio-Connect can provide FBS from a variety of geographies to suit both budgetary and research requirements from suppliers like Life Science Group and Capricorn Scientific. Contact our technical support for more information and availability.

FBS Replacement
Life Science Group also offers FBS Replacement, which is 100% animal component free and is the ideal replacement for FBS, offering a cost effective alternative to serum-based cell culture supplements. FBS Replacement is manufactured from platelet units obtained from healthy human blood donors.

The 3 Rs: Reduction, Refinement and Replacement

Across the world, different initiatives are set up to reduce the amount of animals used in research. One of the steps is reducing the use of animals in production of research reagents. For example, monoclonal antibodies, in contrast to polyclonal antibodies, only use animals in the initial production step and can thereafter be produced without animals. Bio-Connect offers monoclonal antibodies for in vivo research from Bio X Cell, which originate from a totally animal-free facility and are therefore free from infectious diseases.

The next step is to produce research reagents without the use of animals entirely. Bio-Connect offers a wide selection of these products, such as AdipoGen’s recombinant monoclonal antibodies developed from a human antibody phage display library and produced in mammalian cells (CHO or HEK 293). We also offer PromoKine cytokines and growth factors produced by molecular farming using barley grain or tobacco plants as production host.

Carbosynth, the source for carbohydrates and nucleosides

Carbosynth offers an impressive product portfolio including rare or otherwise unobtainable range of over 5000 carbohydrates and nucleosides. The carbohydrate range consists of mono and oligosaccharides, chiral sugars, carbohydrate detergents and enzyme substrates, and the nucleoside range comprises nucleosides, nucleotides, nucleic acids and phosphoramidites.

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New Product: anti-mouse CD172a (SIRPα)

The P84 monoclonal antibody reacts with Signal-Regulatory Protein α (SIRPα), also known as CD172a. SIRPα is a type I transmembrane glycoprotein expressed on monocytes, macrophages, and dendritic cells. Neurons and other tissues of the central nervous system have also been shown to express SIRPα. Its ligand, CD47 is expressed by a wide variety of cells. SIRPα and CD47 regulate dendritic cell-mediated T cell activation, neutrophil migration, and phagocytosis. SIRPα diffuses laterally on the macrophage membrane and accumulates at a phagocytic synapse to bind CD47 which inhibits phagocytosis by macrophages. Anti-SIRPα antibodies that block the interaction of SIRPα with CD47 have been shown to suppress tumor formation in mice. The P84 antibody has been shown to have neutralizing activity in vivo and in vitro.

This antibody is useful for:
• in vivo SIRPα blocking
• in vitro SIRPα blocking
• Western blot
• Immunoprecipitation
• Flow Cytometry

Remember to also order the recommended isotype control and dilution buffer.

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Jackson ImmunoResearch launches Secondary Antibodies for VHH discovery

Anti-Alpaca IgG, Subclass and VHH domain secondary antibodies to optimize VHH (single-domain) antibody development and application.

The new Jackson ImmunoResearch Anti-Alpaca secondary antibody product line targets the IgG repertoire of camelid immunoglobulins, including whole molecule, subclass and VHH domain specificities. They are raised against alpaca and cross-react with llama.

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New unique products by AdipoGen

AdipoGen Life Sciences has released a number of unique high quality products and kits this year that might boost your research as well.

  • Erythroferrone ELISA kit (CatNo: AG-45B-0014-KI01) for detection of human Erythroferrone (Myonectin; CTRP15) in serum, plasma and cell culture supernatant. There is no other sensitive and specific kit on the market!
  • Human APRIL and BAFF ELISA kits are the best-in-class. Read more »
  • Gasdermin D reagents: new research tools, incl. Gasdermin D (mouse) ELISA kit, for Pyroptosis & Inflammasome Detection. Read more »
Contact the Bio-Connect customer service for a quote for these brand new products.

Integrins and Integrin Antibodies

Integrins are a family of transmembrane receptor proteins, connecting the cytoskeleton and extracellular matrix (ECM). They bind to ECM glycoproteins including collagens, fibronectins, laminins, and a number of cellular receptors. In addition, integrins function as signal transducers. The different integrins activate differential intracellular signaling pathways that control biological and cellular functions including cell adhesion, migration, proliferation, cell differentiation and apoptosis.

Atlas offers antibodies targeting integrin alpha and beta subunits. The integrin antibodies have been evaluated in one or more of the IHC, WB and ICC-IF applications.

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EnviroLogix, GMO and mycotoxin testing

EnviroLogix is a global leader in the development of rapid GMO and mycotoxin detection technology.

Bio-Connect only supplies the EnviroLogix products in the Netherlands.

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BioVendor, research and diagnostics products

BioVendor is specialized in the development of original immunoassay kits for the detection of novel hormone-like proteins and protein biomarkers as well as related antibodies and recombinant proteins.

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Improve residual endonuclease assays with a new EndonucleaseGTP™ ELISA Kit!

Are you using an endonuclease enzyme to eliminate all host cell nucleic acids and plasmid DNA during your viral vector or vaccine manufacturing process? Do you know if your purification process removes residual endonuclease adequately?

Cygnus Technologies’ NEW EndonucleaseGTP ELISA Kit is the most sensitive assay (LOD ~0.06 ng/ml; LLOQ ~0.31 ng/ml) to detect and quantify residual endonuclease impurity in recombinant viral vector and vaccine preparations. Qualified for assay precision, sensitivity, and accuracy, it is suitable for the detection and quantification of Benzonase* Nuclease and DENARASE* enzymes in < 2 hours.

Don’t risk the safety of your novel gene therapy drug to a process-related impurity. Learn more about Cygnus Technologies’ NEW EndonucleaseGTP ELISA Kit!

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New Product: anti-mouse Ly6G/Ly6C

The NIMP-R14 monoclonal antibody is specific for murine neutrophils. The antibody is reported to react strongly with mouse Ly6G and Ly6C previously referred to as GR-1. Ly6G is a 21-25 kDa member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Ly6G is expressed differentially during development by cells in the myeloid lineage including monocytes, macrophages, granulocytes, and neutrophils. Monocytes typically express Ly6G transiently during development while mature granulocytes and peripheral neutrophils retain expression making Ly6G a good cell surface marker for these populations. Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma and is thought to play a role in the development and maturation of lymphocytes. The NIMP-R14 antibody has been shown to be useful for depletion of neutrophils in mice.

This antibody is useful for:
• in vivo neutrophil depletion
• Immunohistochemistry (paraffin)
• Immunohistochemistry (frozen)
• Immunofluorescence
• Flow Cytometry

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Immune checkpoint inhibitors to treat cancer

Immune checkpoint inhibitory checkpoint molecules are targets for the treatment of cancer and other diseases. Bio-Connect offers products related to immune checkpoints from several suppliers such as antibodies, assays, proteins, primers, cDNA ORF, siRNA/ShRNA and more.

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PromoCell's Primary Cancer Culture System

To study cancer it is very important to culture these cells. Promocell has a complete cell culture solution designed for the selective culture of malignant cells derived from primary tumors or patient-derived xenografts. 

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Introducing Cell Guidance Systems

Cell Guidance Systems develops therapeutic products as well as medical research tools and technologies that expand the possibilities of life science research and medicine. They work closely with many researchers from around the world to develop truly innovative products that address unmet medical and research needs. Many of their products are groundbreaking: For example, PODS™ is overcoming stability issues of many proteins, offering new possibilities in research and hope for new therapies, and ETS-embryo medium enables the production of "artificial embryos" from stem cells.

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New Product: anti-mouse FasL (CD178)

The MFL3 monoclonal antibody reacts with mouse Fas Ligand (FasL) also known as CD178, CD95 Ligand, and TNFSF6. FasL is a 40 kDa type II transmembrane glycoprotein and a member of the TNF superfamily. FasL is expressed on activated T cells and in spleen, testis, and eye. Upon binding to its receptor CD95 (Fas) FasL induces apoptotic cell death to maintain peripheral tolerance. Some tumors over-express FasL and induce the apoptosis of infiltrating lymphocytes, allowing the tumor to escape the effects of an immune response. CD178/CD95 interactions are also thought to play a role in the proliferation of CD8+ cells and neutrophil extravasation, chemotaxis and survival. The MFL3 antibody has been reported to block CD178/CD95 induced apoptosis.

This antibody is useful for:
• in vivo FasL blockade
• in vitro FasL blockade
• Functional assay
• Immunohistochemistry (paraffin)
• Flow Cytometry

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Immune Checkpoints

Working in the field of Immune checkpoints? Bio-Connect offers products related to immuneckpoints from several suppliers such as antibodies, assays, proteins, primers, cDNA ORF, siRNA/ShRNA and more. On this page you will find popular target links to products and background information. If you have any questions or if you are looking for certain products you can contact our techsupport.

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Cell Guidance Systems Reagents and Services

Bio-Connect has become distributor for the products of Cell Guidance Systems in the Benelux. Their reagents and services enable control, manipulation and monitoring of the cell, both in-vitro and in-vivo.

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NxtGen 3D Tumorsphere Medium XF

The PromoCell 3D Tumorsphere Medium XF is a culture system developed for the standardized serial culture of cancer cell lines as tumorspheres/mammospheres. The formulation supports sustained cell proliferation allowing for serial passage of the 3D culture. Its serum-free and xeno-free formulation provides a culture environment devoid of all stimuli originating from non-defined materials. Tested with a variety of cell lines including U-87 MG, MCF-7, MDA-MB-231, HT-29, HT1080, HepG2, A-549, PAnc-1, LNCaP and A-431.

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Easy single-cell passage of human ES and iPS cells without rock inhibitors

Biolaminin 521 LN (LN521) is a full-length, human, recombinant laminin 521 cell culture substrate. It provides an optimal environment for feeder-free culture of human PSCs, MSCs and most anchoragedependent progenitor cell types under completely chemically defined, animal component-free conditions. LN521 renders a single-cell passage hPSC culture system that is medium independent and offers a weekend-free protocol. Importantly, the cells behave predictably, are homogenously pluripotent and karyotypically stable.

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Stem Cell Applications

OZ Biosciences supplies several solutions for stem cells applications:
• Enhancement of viral transduction - ViroMag, ViroMag R/L, AdenoMag
• Highly efficient DNA transfection - DreamFect™ Stem
• Easy mRNA transfection - RmesFect™ Stem
• Delivery of siRNA or miRNA - Lullaby™ Stem
• Transport of recombinant proteins – Pro-DeliverIN™ and Ab-DeliverIN™
• Senescence detection – Senescence kit
• Integrated Magnetic Immuno-Cell Sorting and Transduction - Viro-MICST™

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Netrin-1 for Generation of iPS Cells

Netrin-1 seems to be the 5th Element of classical iPS cell factors. Together with Netrin-1 the original factors Oct4, Sox2, Klf4 and c-Myc can produce 15 times more induced pluripotent stems cells (iPSCs) under specific culture conditions. This treatment did not affect the quality of cell reprogramming. Further research is ongoing for a better understanding of the mode of action of this protein in stem cell physiology to accelerate the development of clinically useful iPS cells.

AdipoGen Life Sciences provides Stable & Active Netrin-1 Proteins, described in: "Netrin-1 regulates somatic cell reprogramming and pluripotency maintenance" D. Ozmadenci, et al.; Nat. Commun. 6, ID7398 (2015)

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Glial-Mag transfection kit the new top selling product

Glial-Mag is designed to reach optimal transfection efficiency with microglial cell lines and primary cells. Glial-Mag kit is the association of a specific magnetic nanoparticles formulation (Glial-Mag reagent), issued from OZ Biosciences Magnetofection™ technology and a booster (Glial-Boost) designed to enhance transfection efficiency.

“Magnetofection is superior to other chemical transfection methods in a microglial cell line.” Smolders S. et al - J Neurosci Methods. 2018

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Design your own vector using VectorBuilder's platform

• Free online vector design platform
• Custom vectors
• Virus packaging
• BAC modification
• Mutagenesis

15% Off your first order when using promocode: "BioConnect_15%Off_2020" at

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AdipoGen Product Highlights

AdipoGen Life Sciences has many quality products:
• IL-36gamma ELISA Kit (AG-45B-0008): Biomarker for Psoriasis
• VISTA (mouse):-COMP (mouse) (rec.) (His) (AG-40B-0181): An active form of a new Immune Checkpoint Protein
• anti-BAFF (mouse), mAb (blocking) (Sandy-2) (AG-20B-0063): Unique - Blocks BAFF activity in mice (in vivo)
• PD-1 (mouse) mAb (blocking) (1H10) (AG-20B-0075): Blocks PD-1 binding. Induces a rapid Activation and Proliferation of T Cells
• IL-1alpha (p18)(mouse) mAb (Teo-1) (AG-20B-0064): To detect cleaved mouse IL-1alpha
• NLRP3/NALP3 mAb (Cryo-2) (AG-20B-0014): Key Inflammasome Antibody

New Product: anti-mouse VISTA monoclonal antibody

This antibody is useful for in vivo and in vitro blocking of VISTA signaling
Identifying VISTA expressing cells via flow cytometry

Why is VISTA signaling important?
VISTA is a recently discovered immune checkpoint protein found at high levels on myeloid cells that infiltrated tumors. Preclinical studies with VISTA blocking antibodies have shown promising improvements in anti-tumor T-cell responses. As always Bio X Cell antibodies are specifically formulated for in vivo use and feature:

• > 95% purity
• Ultra-low endotoxin levels
• Preservative, stabilizer, and carrier protein-free

Anti-mouse VISTA is available in both InVivoMab and InVivoPlus formats!

Zebrafish-Validated Antibodies

As the leading manufacturer of zebrafish antibodies, GeneTex offers an extensive portfolio of reagents validated for use in this model system. GeneTex currently offers a continuously expanding catalog of reagents designed expressly for zebrafish research, with more than 450 newly developed zebrafish antibodies. These antibodies havebeen tested on zebrafish samples in a variety of applications including, but not limited to, immunofluorescence, immunohistochemistry, and western blot.

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New Product: InVivoMAb anti-mouse CXCL9 (MIG)

Bio X Cell just introduced the new anti-mouse CXCL9 (MIG) (CatNo#BE0309). Formulated for in vivo use.

• Ultra-low endotoxin levels
• Greater than 95% purity
• High concentration (3-10 mg/ml)
• Formulated in preservative-free, carrier protein-free, and amine-free buffer

Available in 1, 5, 25, 50 and 100 mg sizes!

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Rabbit Monoclonal Antibodies

Antibodies raised in rabbits have distinct advantages over antibodies produced in mice, including having a higher affinity and the rabbit immune-response is able to recognize epitopes that are non-immunogenic in mice. Thus, rabbit monoclonals are more specific and more sensitive antibodies than those produced in mouse. The rabbit monclonals are ideal for applications like IHC, IF staining and flow cytometry.

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Helix-IN™ DNA Transfection Reagent

Helix-IN™ DNA Transfection Reagent opens up new possibilities for addressing issues of classical transfection technologies. OZ Biosciences revolutionizes Polyfection with the design of Helix-IN™, a novel patented Cationic Hydroxylated Amphiphilic Multi-block Polymer (CHAMP™ Technology). This novel bi-functional co-polymer is biocompatible, ionizable, pH responsive and biodegradable.

Cellular stress is reduced, viability preserved and efficiency enhanced.

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ProSpec new in our portfolio

We would like to inform you that we have become the distributor of ProSpec-Tany TechnoGene.

ProSpec is a leading biotechnology company with over 20 years of extensive experience in recombinant protein production which result in a robust protein portfolio. With more than 6000 recombinant proteins, peptides and antibodies. The proteins are rigorously tested to meet the research and development demand for excellent quality and uncompromising biological activity.

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Mebiol® Gel PNIPAAm-PEG 3D Thermoreversible Hydrogel

Mebiol® Gel PNIPAAm-PEG 3D Thermoreversible Hydrogel from Cosmo Bio. PNIPAAm-PEG a block co-polymer of poly(N-isopropylacrylamide) and poly(ethylene glycol) for 3D Cell Culture and other applications.

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Conjugated, Alpaca and Mass-Spec validated antibodies

Conjugated monoclonal antibodies for direct Western blot or staining eliminates need for secondary antibodies. However Alpaca secondary antibodies creating new possibilities in multiple labelling assays. Unique Mass Spec validated primary antibodies are also available.

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New Product: InVivoMAb anti-mouse CD16/CD32

Bio X Cell just introduced the new anti-mouse CD16/CD32 antibody, clone 2.4G2 (CatNo#BE0307). Perfect for use in flow cytometry staining to block binding of IgG to Fc receptors. Available in 5, 25 50, and 100 mg sizes!

The 2.4G2 monoclonal antibody reacts specifically with mouse CD16 (FcγRIII) and CD32 (FcγRII). It has also been reported to react non-specifically via its Fc domainto FcγRI. CD16 and CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. These receptors bind to the Fc portion of antibody-antigen complexes and play a role in adaptive immune responses. The 2.4G2 antibody is commonly used in flow cytometry staining experiments to prevent non-specific binding of IgG to the FcγIII and FcγII, and possibly FcγI, receptors prior to staining with antigen specific primary antibodies. The Fab fragments of the 2.4G2 antibody have also been used to block Fc receptors in vivo.

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Need for Primary Cell Culture Media?

Does your current supplier has delivery problems, will you start a new project with primary cells or just simply want to use high quality media? Use PromoCell's media for primary cells or stem and blood cells!

High-quality medium is vital for successful cell culture. Therefore, PromoCell adheres to strict quality standards and produces its medium only from raw materials from strictly approved sources. They also have invested a lot of effort in media optimization and have substituted serum by defined substances wherever possible. This ensures effective cell growth and avoids the unpredictable effects of indeterminate compounds.

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Notch Signaling Pathway

The Notch pathway plays an important role in many different processes in a wide range of tissues and deregulations in Notch signaling components have been associated with various human disorders such as cancer, immune disorders, developmental syndromes, stroke and cognitive symptoms. Other disorders affecting vertebral column such as scoliosis or the vasculature, hypertension and the developmental disorder Alagille syndrome are also caused by Notch defects.

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Protein extracts, lysates and tissue arrays

The availability of tissues to study proteins can be limited. We have the solution! We can provide you with total or membrane protein extracts / lysates from different tissues and species and with an extensive and unique collection of frozen tissue sections, paraffin tissue sections, and tissue arrays for human normal, diseased, and tumor tissue types.

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The BlotBot, an automated blot processor

If you are incubating and washing gels and blots on a regular basis and want to increase your productivity use the Freedom Rocker™ BlotBot®!

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Food Industry

Bio-Connect has an extensive portfolio of research products including antibodies, assay kits, bio-chemicals (e.g. inhibitors and standards), antibiotics, proteins, enzymes, biological samples, qPCR mixes, products for sample preparation/purification and food analysis, food safety tests and many more for different research area’s including metabolism, obesity, diabetes and food.

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6th edition of Stelvio for Life

On Saturday the 2nd of September, the 6th edition of Stelvio for Life took place. More than 300 participants ran, cycled or walked the custom trail which was adjusted due to the heavy snowfall and cold. For safety, it was decided to reach a turning point after 13 km at 2147 meters. After that, participants had to descend as opposed to the original edition finishing after 21.1 km at 2,758 meters. The distance was extended to 26 km and both the start and finish were on the village square in Bormio, where all our participants finished safely.

The participants collected almost € 600,000 for personalized cancer research. We would like to especially thank our Bio-Connect participants and of course our sponsors!

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Loading Controls GeneTex

Loading controls are used to ensure that equal amounts of each protein sample are loaded into the wells of an SDS-PAGE gel. Selecting a proper loading control whose signal remains constant under the tested conditions is essential in order to properly interpret the results of the western blot experiment.

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Antibodies to study Neurodegenerative Disorders

We offer an excellent catalog of high-quality antibodies from GeneTex to study the processes involved in neurodegenerative disorders (such as: Alzheimer's disease, Parkinson's disease, Huntington's disease, Amyotrophic lateral sclerosis and prion diseases), the majority of which are validated for multiple applications.

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Atlas Antibodies in Neuroscience

The Atlas catalog offers a wide range of neuroscience antibodies, validated in neuro tissues and cell lines. Atlas Antibodies offers their neuroscience antibodies divided in five panels: antibodies involved in signaling, neural lineage markers, aging, neurodegenerative disorders and developmental processes.

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New Product: Magnetofectamine O2 Transfection Kit

Magnetofectamine™ O2 is the new powerful and ideal transfection kit designed for primary and hard-to-transfect cells. The alliance of MTX transfection reagent and CombiMag leads to increased transfection efficiency, minimized toxicity and enhanced gene expression. Less nucleic acids used - Minimized toxicity. Reference.

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Bio X Cell bulk antibodies for in vivo research

With over 300 monoclonal antibodies covering a diverse range of research areas Bio X Cell is your source for purified bulk antibodies. Their reagent portfolio is specialized towards antibodies which are widely used for in vivo and in vitro antigen neutralization and pathway blockade as well as cell specific depletion. All Bio X Cell products are significantly discounted for customers from academia.

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HYPE-293 transfection reagent for Bioproduction

The new HYPE-293™ Transfection Kit of OZ Biosciences is dedicated to achieve High Yield Protein Expression in HEK-293, 293-S, 293-F or any 293-related cells growing in suspension. Scale-up to larger volumes for production of milligrams of protein per liter of cell culture is straightforward and easy with simple and cost efficient handling steps.

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New range of reverse transcription reagents

Solis BioDyne new FIREScript Reverse Transcriptase is a genetically engineered MMLV (Moloney Murine Leukemia Virus) based reverse transcriptase.

• 30 days stability at room temperature
• Fast and efficient, comparable to the best enzymes on the market
• Different formats available (mixes and kits)
• High working temperature up to 50°C
• High reproducibility
• For in vitro use only

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Checkit instant pipette verification

Verification on the spot. Evaluate the accuracy of your pipettes in seconds with Next Advance convenient Pipette Checkit® cartridges. Low-cost, fast, and easy to use, the Checkit can save money on calibration services while giving you the confidence that your pipettes are delivering accurate volumes. No more trying to read a tiny droplet’s weight on a high-precision balance.

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Immunometabolism & Inflammation research

AdipoGen Life Sciences develops and manufactures new products in the areas of cancer, immunology, (neuro)inflammation, metabolic syndrome (diabetes, obesity), stem cell biology and neurodegeneration. Major product lines are ELISA kits, (recombinant) antibodies, proteins, natural products and small molecules. AdipoGen is the expert in Inflammasome Signaling.

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Stelvio for Life

September 2nd 2017 the foundation Stelvio for Life organizes for the 6th time the climbing of one of the highest paved, most beautiful and most challenging mountains in Europe, the Stelvio Pass in Northern Italy.

If you also want to support our participants, please make a donation to the Bio-Connect team.

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GeneTex engages CRISPR/Cas9 technology to validate antibody specificity

At GeneTex, they continue to provide the research community with the highest quality and most stringently validated antibodies by engaging CRISPR/Cas9-mediated knockout technology. In a collaborative effort with EdiGene, a company that generates knockout or genome-edited cell lines using an innovative CRISPR/Cas9 methodology, GeneTex will use lysates made from these cell lines for western blot assays to confirm target specificity of their antibodies. This constitutes the “Knockout” arm of their "Knockout/Knockdown validation" initiative.

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Sino Biological proteins, antibodies, genes, and ELISA kits

Sino Biological manufactures high quality research reagents for basic, translational, and clinical research. Their portfolio includes proteins, antibodies, cDNAs, qPCR primers and ELISAs with a focus on immune checkpoints, targeted and immunotherapy, virology, cytokines, CD antigens, FC receptors, enzymes and kinases, growth factors, signal transduction, and biomarkers.

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Bio-Connect your antibody supplier

Whether you are looking for a good antibody against your target, your current antibody is discontinued, out of stock or simply not working do not worry! Bio-Connect can help you to secure your supply of antibodies to continue your research. See here our antibody suppliers with their antibody strengths.

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AdipoGen ELISA Kits

AdipoGen ELISA kits for key biomarkers for metabolic syndrome, inflammatory diseases and cancer & autoimmune diseases. AdipoGen Life Sciences is the original manufacturer of many widely used and cited ELISA kits. AdipoGen Life Sciences is continuously developing ELISA kits for new targets which are of interest to the research community. The AdipoGen Life Sciences ELISA Kits allow reproducible results with low inter- and intra-assay variation, show high sensitivity and can be used for a broad range of sample types.

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SeqPure PCR Purification System

Biochain’s SeqPure PCR Purification System is designed for rapid and efficient purification of PCR products. The system utilizes paramagnetic bead technology to selectively bind DNA fragments 100 bp and larger in size. Simple washing steps will remove salts, primers, primer-dimers, nucleotides, and enzymes prior to elution of the purified PCR product. The SeqPure DNA Purification System is ideal for purification of DNA using either manual procedures or automated liquid handling instruments.

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Dual Role of TGF-β in Cancer Promotion and Suppression

Transforming growth factor (TGF)-beta signaling can have either tumor-suppressing or tumor-promoting effects in a cell- and context-dependent manner. The canonical Smad pathway is responsible for most of the TGF-β biological responses leading to tumor suppression (growth arrest, apoptosis, and prevention of immortalization) and tumor promotion (EMT, migration, invasion, and metastasis).

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Caspase Detection

A central component of the apoptotic process is a cascade of proteolytic enzymes called caspases. Caspases participate in a series of reactions that are triggered in response to pro-apoptotic signals and result in the cleavage of protein substrates, causing the disassembly of the cell. Caspases have been identified in organisms ranging from... Read more »»

Merck provides a wide range of caspase antibodies, inhibitors, proteins and assays to help detect and study caspase activation in apoptotic cells.

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Linking NAD+ Signaling & Cancer

Many and various NAD+-dependent signaling pathways regulate fundamental processes such as transcription, DNA repair, cell cycle progression, apoptosis, proliferation, survival and metabolism, which are linked to cancer development. NAD+-dependent signaling reactions involve the degradation of NAD, therefore permanent nucleotide resynthesis through different biosynthetic pathways is crucial for incessant cancer cell proliferation. Targeting the NAD metabolism, its synthesis and signaling pathways (PARPs, SIRTs, MARTs, CD38) show several effects on cancer cells and are therefore new and promising concepts for cancer treatment.

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Excellent PTEN Antibody

Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN is best known as a negative regulator of PI3K/AKT signaling by dephosphorylating PIP3. However, its functional shadow is now known to extend beyond PI3K/AKT pathway inhibition to include PIP3-independent roles affecting cell migration and genomic integrity. In addition, loss of normal PTEN activity has repercussions not only for tumor cell proliferation but also for regulation of the tumor microenvironment. Thus, there are clearly new frontiers to be explored in the PTEN field.

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Atlas Antibodies in Breast Cancer Research

Atlas is very active in the field of breast cancer research. A wide range of their antibodies are used by scientists working to understand breast cancer. A selection of polyclonal and monoclonal antibodies directed towards protein targets interesting for breast cancer research are P53, HER2, CD44, PGR and Ki-67.

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Santa Cruz Biochemicals

Santa Cruz Biotechnology offers over 175,000 specialty biochemicals under the ChemCruz® brand. Their protein-interacting inhibitors, activators and substrates are designed to work seamlessly with primary antibodies to study protein function. They also offer more generalized chemicals such as prepared buffers, detergents and solvents that are suitable for everyday laboratory use.

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